Laila A Alhadad
Kuwait University, Kuwait
Title: Targeting ovarian cancer using cholesterol conjugated HSP27 inhibitor encapsulated into nano low density lipoprotein particles
Biography
Biography: Laila A Alhadad
Abstract
Cholesterol conjugated to anti-cancer moiety has an effective drug delivery for cancer therapy. Many studies developed new promising cholesterol dug conjugates for either disease diagnosis or cancer treatment. In our research laboratory, we synthesized cholesterol chemically conjugated to a potent anti-cancer moiety that targets HSP27 and reduces HER2 stability through HER2 pathway because of their cellular importance. Both proteins are proportionally up and down regulated in cancerous cells and cause chemotherapy drug resistance. Therefore, synthesis of cholesterol conjugated to anti-cancer agent target both proteins is an attractive approach. Normally, cholesterol esters transport to and from the cells via lipoproteins synthesized in the liver. Based on that, first we designed, synthesized and characterized cholesterol conjugated HSP27 inhibitor. Second, measured the particle size of the drug encapsulated into LDL using DLS technique. Third, tested SKOV3 cell line viability with the nano-LDL particles containing cholesterol conjugate using MTT assay. In sum, the agent which loaded into nano-LDL particles, showed potency inhibiting SKOV3 cell's growth. In sum, development of new medicinal compounds for cancer treatment became one of the principle attractive approaches in pharmaceutical chemistry.